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Isolation of linoleic and α‐linolenic acids as COX‐1 and ‐2 inhibitors in rose hip

Author:
Jäger, A. K., Petersen, K. N., Thomasen, G., Christensen, S. Brøgger
Source:
Phytotherapy research 2008 v.22 no.7 pp. 982-984
ISSN:
0951-418X
Subject:
fractionation, high performance liquid chromatography, linoleic acid, osteoarthritis, silica
Abstract:
Rose hip has previously shown clinical efficacy in the treatment of osteoarthritis, and organic solvent extracts of rose hip have showed inhibition of cyclooxygenase‐1 and ‐2. A petroleum ether extract of rose hip was fractioned by VLC on silica; on a C‐18 column and by HPLC. Each step was COX‐1/2 activity‐guided. The bioassay‐guided fractionation led to the isolation of linoleic acid (the IC₅₀ for COX‐1 was 85 µm and 0.6 µm for COX‐2) and α‐linolenic acid (the IC₅₀ for COX‐1 was 52 µm and 12 µm for COX‐2). The COX‐2/COX‐1 ratio was 0.007 for linoleic acid and 0.2 for α‐linolenic acid. Linoleic acid and α‐linolenic acid contribute to the COX‐1 and ‐2 inhibitory activity of rose hip. Copyright © 2008 John Wiley & Sons, Ltd.
Agid:
507733