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A two-stage oxygen supply control strategy for enhancing milk-clotting enzyme production by Bacillus amyloliquefaciens
- Ding, Zhongyang, Ai, Lianzhong, Ouyang, Anran, Ding, Mingliang, Wang, Wangfei, Wang, Boda, Liu, Shuangping, Gu, Zhenghua, Zhang, Liang, Shi, Guiyang
- European food research & technology 2012 v.234 no.6 pp. 1043-1048
- Bacillus amyloliquefaciens, cell growth, enzymes, fermentation, milk clotting, oxygen
- To enhance the yield and productivity of milk-clotting enzyme (MCE) by Bacillus amyloliquefaciens, a two-stage oxygen supply control strategy was proposed and successfully applied in the MCE fermentation. During the first 16 h, KLa was controlled at 72.2 h−1 to obtain high cell growth rate (v) and MCE activity (MCA) productivity (r MCA). Subsequently, KLa was controlled at 33.9 h−1 to maintain high specific MCA productivity (q MCA). Using this strategy, MCA peaked at 36 h with the MCA of 6,590.41 SU ml−1, which was 18 h earlier than other investigated processes. The concept and results described represent the basis of an industrial scale-up process to achieve high MCE yield, MCA productivity and MCA/proteolytic activity.