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Butanol Production from Crystalline Cellulose by Cocultured Clostridium thermocellum and Clostridium saccharoperbutylacetonicum N1-4

Nakayama, Shunichi, Kiyoshi, Keiji, Kadokura, Toshimori, Nakazato, Atsumi
Applied and environmental microbiology 2011 v.77 no.18 pp. 6470-6475
Clostridium acetobutylicum, Clostridium beijerinckii, Clostridium cellulolyticum, Clostridium saccharoperbutylacetonicum, Clostridium thermocellum, butanol, cellulose
We investigated butanol production from crystalline cellulose by cocultured cellulolytic Clostridium thermocellum and the butanol-producing strain, Clostridium saccharoperbutylacetonicum (strain N1-4). Butanol was produced from Avicel cellulose after it was incubated with C. thermocellum for at least 24 h at 60°C before the addition of strain N1-4. Butanol produced by strain N1-4 on 4% Avicel cellulose peaked (7.9 g/liter) after 9 days of incubation at 30°C, and acetone was undetectable in this coculture system. Less butanol was produced by cocultured Clostridium acetobutylicum and Clostridium beijerinckii than by strain N1-4, indicating that strain N1-4 was the optimal strain for producing butanol from crystalline cellulose in this coculture system.