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Development and characterization of EST-derived microsatellite makers for swimming crab, Portunus trituberculatus
- Wei, Qian, Wang, Chunlin, Mu, Changkao, Song, Weiwei, Li, Ronghua
- Conservation genetics resources 2013 v.5 no.2 pp. 511-513
- Portunus trituberculatus, alleles, coastal water, crabs, expressed sequence tags, heterozygosity, loci, microsatellite repeats, swimming, China
- In this study, sixteen polymorphic microsatellite makers were developed and characterized from expressed sequence tag sequence of the swimming crab, Portunus trituberculatus. The analysis of polymorphic was performed by using 30 specimens from the coastal waters of Xiangshan, Zhejiang province, China. The number of alleles at each locus ranged from 4 to 14 with an average of seven alleles per locus. The ranges of observed and expected heterozygosity were from 0.517–1.000 to 0.508–0.736, respectively. The polymorphism information content ranged from 0.375 to 0.710. Four loci were found deviate significantly from Hardy–Weinberg equilibrium. These microsatellite loci will be helpful for the genetic evaluation of P. trituberculatus enhancement.