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Morphological characterization and immunohistochemical detection of the proinflammatory cytokines IL-1β, IL-17A, and TNF-α in lung lesions associated with contagious bovine pleuropneumonia

Sterner-Kock, Anja, Haider, Wolfram, Sacchini, Flavio, Liljander, Anne, Meens, Jochen, Poole, Jane, Guschlbauer, Maria, Heller, Martin, Naessens, Jan, Jores, Joerg
Tropical animal health and production 2016 v.48 no.3 pp. 569-576
Mycoplasma mycoides subsp. mycoides, cattle, contagious bovine pleuropneumonia, histopathology, immune response, immunohistochemistry, inflammation, interleukin-17, interleukin-1beta, lungs, macrophages, necrosis, neutrophils, tumor necrosis factor-alpha, vasculitis
Contagious bovine pleuropneumonia (CBPP), a severe respiratory disease, is characterized by massive inflammation of the lung especially during the acute clinical stage of infection. Tissue samples from cattle, experimentally infected with Mycoplasma mycoides subsp. mycoides Afadé, were subjected to histopathological and immunohistochemical examination in order to provide insight into innate immune pathways that shape inflammatory host responses. Lung lesions were characterized by vasculitis, necrosis, and increased presence of macrophages and neutrophils, relative to uninfected animals. The presence of three cytokines associated with innate inflammatory immune responses, namely, IL-1β, IL-17A, and TNF-α, were qualitatively investigated in situ. Higher cytokine levels were detected in lung tissue samples from CBPP-affected cattle compared to samples derived from an uninfected control group. We therefore conclude that the cytokines TNF-α and IL-1β, which are prevalent in the acute phase of infections, play a role in the inflammatory response seen in the lung tissue in CBPP. IL-17A gets released by activated macrophages and attracts granulocytes that modulate the acute phase of the CBPP lesions.