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Enzymatic hydrolysis of different allomorphic forms of microcrystalline cellulose

Ciolacu, Diana, Gorgieva, Selestina, Tampu, Daniel, Kokol, Vanja
Cellulose 2011 v.18 no.6 pp. 1527-1541
Aspergillus niger, Hypocrea jecorina, X-ray diffraction, biodegradation, cellulose, crystal structure, endo-1,4-beta-glucanase, enzymatic hydrolysis, hydrolysis, microstructure, particle size, reducing sugars, scanning electron microscopy
This paper investigates the enzymatic hydrolysis of three main allomorphic forms of microcrystalline cellulose using different cellulases, from Trichoderma reesei and from Aspergillus niger, respectively. It was demonstrated that both the morphological and crystalline structures are important parameters that have a great influence on the course of the hydrolysis process. The efficiency of the enzymatic hydrolysis of cellulosic substrates was estimated by the amounts of reducing sugar and by the yield of the reaction. Changes in the average particle sizes of the cellulose allomorphs were determined during enzymatic hydrolysis. The accumulation of soluble sugar within the supernatant was used as a measure of the biodegradation process’s efficiency, and was established by HPLC-SEC analysis. Any modifications in the supramolecular structure of the cellulosic residues resulting from the enzymatic hydrolysis were determined by X-ray diffraction. The action of each cellulase was demonstrated by a reduction in the crystalline index and the crystallite dimensions of the corresponding allomorphic forms. The crystalline structure of allomorphic forms I and II did not suffer significant modifications, while cellulose III recorded a partial return to the crystalline structure of cellulose I. The microstructures of cellulose allomorph residues were presented using optical microscopy and scanning electron microscopy.