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Effect of Inonotus obliquus enriched diet on hematology, immune response, and disease protection in kelp grouper, Epinephelus bruneus against Vibrio harveyi

Harikrishnan, Ramasamy, Balasundaram, Chellam, Heo, Moon-Soo
Aquaculture 2012 v.344-349 pp. 48-53
Epinephelus bruneus, Inonotus, Vibrio harveyi, albumins, antibacterial properties, diet, erythrocytes, fish feeding, globulins, health status, hematology, hemoglobin, immune response, lymphocytes, mortality, mushrooms, protein content, weight gain
The aim of the present study was to determine the changes in the hematological and immunological response in kelp grouper Epinephelus bruneus against Vibrio harveyi infection after fed with 0%, 0.1%, 1.0%, and 2.0% Chaga mushroom, Inonotus obliquus extract enriched diet. All the doses significantly influenced the weight gain (WG) from weeks 1 to 4 when compared to the control. Other determined growth performance did not have any significant change at any time, except protein efficiency ratio in 2.0% diet as compared to control. The white blood cell and red blood cell levels, hemoglobin, haematocrite, lymphocytes, and monocytes significantly increased in infected fish fed with all diets. Total protein contents, albumin, and globulin level significantly increased with 1.0% and 2.0% than those of 0.1% diet from weeks 1 to 4. The infected fish fed with 1.0% and 2.0% diet significantly enhanced phagocytic activity from weeks 1 to 4. The respiratory burst and anti-protease activities significantly enhanced in infected fish fed with any doses of enriched diet on weeks 2 and 4. The lysozyme activity and serum bactericidal activity significantly increased in all doses of enriched diet from weeks 1 to 4. The cumulative mortality was low in 1.0% and 2.0% diets with 20% and 15% than those of 0.1% diet. This study suggest that 1.0% and 2.0% doses of diet significantly influence on growth performance and hematology, and could positively enhance innate immune system that effectively promote the health status of kelp grouper against V. harveyi infection.