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Variation of High-Molecular-Weight Secalin Subunit Composition in Rye (Secale cereale L.) Inbred Lines

Salmanowicz, Boleslaw P., Langner, Monika, Kubicka-Matusiewicz, Helena
Journal of agricultural and food chemistry 2014 v.62 no.43 pp. 10535-10541
Secale cereale, acetonitrile, capillary zone electrophoresis, ethylene oxide, inbred lines, polyacrylamide gel electrophoresis, polyethylene glycol, rye
In this study, identification and characterization of the rye HMW secalin subunit (HMW-SS) composition in 68 inbred rye (Secale cereale L.) lines was performed by capillary zone electrophoresis (CZE). The HMW-SS were separated in an uncoated fused-silica capillary using an isoelectric iminodiacetic buffer in combination with poly(ethylene oxide), lauryl sulfobetaine, and acetonitrile as the separation buffer. The separations of the nonalkylated HMW-SS provided very good resolution and high reproducibility. Generally, the x-type rye HMW-SS were more abundant and have longer migration times than the y-type subunits. Both types of rye HMW-SS were separated into the major protein peak and one or two minor peaks. In total, seven x-type HMW-SS, five of which were newly identified subunits, and six y-type subunits, four of which were new, were distinguished on the basis of their CZE migration times. The migration order of the rye HMW-SS using CZE differed considerably from the relative electrophoretic mobilities in the SDS-PAGE gels.