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Production of Bioactive Compounds with Antitumor Activity Against Sarcoma 180 by Pleurotus sajor-caju

Assis Ivaneliza Simionato, Chaves Mariane Bonatti, Silveira Marcia Luciane Lange, Gern Regina Maria Miranda, Wisbeck Elisabeth, Júnior Agenor Furigo, Furlan Sandra Aparecida
Journal of medicinal food 2013 v.16 no.11 pp. 1004-1012
Pleurotus sajor-caju, ammonium sulfate, antineoplastic activity, antineoplastic agents, biomass, bioreactors, calcium carbonate, containers, culture media, exopolysaccharides, glucose, growth retardation, mice, mycelium, pH, peptones, sarcoma, yeast extract
This work studied the influence of culture medium composition and pH on exopolysaccharide (EPS) production by Pleurotus sajor-caju and validates the antitumor activity of the produced EPSs and of the mycelial biomass (intracellular polysaccharides [IPSs]) against Sarcoma 180 (S180) cells. The effect of the initial concentrations of (NH₄)₂SO₄, yeast extract and soy peptone on EPS production by P. sajor-caju was studied in shake flasks. A bioreactor was used to evaluate the pH values and the initial CaCO₃ and glucose concentrations. Extracts of EPSs (PE1) and IPSs obtained through two different separation processes (PM1 and PM2) were tested on mice inoculated with S180 cells. A medium containing 2.5, 1.0, and 1.0 g/L of (NH₄)₂SO₄, yeast extract and soy peptone, respectively, provided the highest EPS concentration (0.6 g/L). The use of pH 4.0, 1.0 g/L CaCO₃ and 20 g/L initial glucose concentration enhanced EPS productivity (3.84 g/L per hour). The PE1 extract promoted the highest reduction of S180 growth (86%), followed by the PM2 extract (80%); growth reduction was dose-independent for both substances. This work provides information about culture medium and conditions that enhanced the production of extracellular polysaccharides by P. sajor-caju. The results can contribute to the search for new bioactive products bringing novel aspects to the medical and pharmaceutical areas.