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Interspecific hybridisation of cytoplasmic male-sterile rapeseed with Ogura cytoplasm and Brassica rapa var. pekinensis as a method to obtain male-sterile Chinese cabbage inbred lines

Kamiński, Piotr, Podwyszyńska, Małgorzata, Starzycki, Michał, Starzycka-Korbas, Elżbieta
Euphytica 2016 v.208 no.3 pp. 519-534
Brassica napus var. napus, Brassica rapa, Chinese cabbage, agronomic traits, backcrossing, cytoplasm, cytoplasmic male sterility, genome, genotype, hybrids, inbred lines, interspecific hybridization, plant breeding, rapeseed, seed productivity, vigor, Poland
During 2011–2014, three self-compatible Chinese cabbage inbred lines (Brassica rapa, genome AA 2n = 20) were sexually hybridised with cytoplasmic male-sterile (CMS Ogu-INRA) and fertile lines of rapeseed (Brassica napus var. oleifera, genome AACC 2n = 38). Interspecific F₁ hybrids and their consecutive back-crosses (BCs) toward B. rapa were obtained, and their morphological and commercial characteristics, as well as their ability for generative propagation, were analysed. Cytometric and morphological analyses confirmed the interspecific character of the F₁ hybrids in comparison with B. napus and B. rapa parental lines. All interspecific F₁ hybrids had intermediate morphological characteristics typical of both parental components in the generative and vegetative stages. The F₁ hybrids of CMS B. napus × B. rapa were sterile, as were those of fertile B. napus × B. rapa. Good seed productivity from CMS B. napus × B. rapa and consecutive back-cross 1 (BC₁)–BC₃ generations with Chinese cabbage as the recurrent parent was obtained. Plants of the BC₁ generation of CMS B. napus × B. rapa were sterile and had highly diversified morphological characteristics, both in the vegetative and generative phases. The BC₃ generation of CMS B. rapa was also sterile and more uniform than BC₁, with good vigour and most of the typical Chinese cabbage agronomic traits. As a result, a collection of Chinese cabbage genotypes having the CMS trait, which is valuable for the breeding of commercial F₁ hybrids, was obtained for the first time in Poland.