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Molecular Detection of Epiphytic Acaryochloris spp. on Marine Macroalgae

Author:
Ohkubo, Satoshi, Miyashita, Hideaki, Murakami, Akio, Takeyama, Haruko, Tsuchiya, Tohru, Mimuro, Mamoru
Source:
Applied and environmental microbiology 2006 v.72 no.12 pp. 7912-7915
ISSN:
0099-2240
Subject:
chlorophyll, epiphytes, gel electrophoresis, genes, macroalgae, plastids, ribosomal RNA
Abstract:
A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.
Agid:
515880