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The effect of ions on the adhesion and internalization of Chlamydia trachomatis by HeLa cells

Author:
Sneddon, John M., Wenman, Wanda M.
Source:
Canadian journal of microbiology 1985 v.31 no.4 pp. 371-374
ISSN:
0008-4166
Subject:
Chlamydia trachomatis, adhesion, barium, calcium, calmodulin, cations, cobalt, glucose, iron, lanthanum, magnesium, manganese, potassium, sodium, strontium, verapamil, zinc
Abstract:
The adhesion and internalization of Chlamydia trachomatis by HeLa cells was unaffected by removal of K⁺, Mg²⁺, or glucose from the incubation medium, slightly reduced by removal of Na⁺, and significantly reduced by omission of Ca²⁺. Sr²⁺, Mg²⁺, and Mn²⁺ could replace Ca²⁺ in the adhesion but only Sr²⁺ supported internalization, and La³⁺, Co²⁺, Fe³⁺, Ba²⁺, and Zn²⁺ all reduced internalization more than adhesion. During initial infection there was no measurable difference in the uptake or release of ⁴⁵Ca²⁺ or ⁸⁶Rb⁺ between infected and noninfected HeLa monolayers. Infection was not prevented by pretreatment of the monolayers with the calcium channel blockers, verapamil, D600, and nitrendipine, or the calmodulin inhibitors, TMB-8 or trifluperazine. The results suggest that divalent cations are not essential for chlamydial infection but that the process of internalization is facilitated by the presence of cations, particularly Na⁺ and Ca²⁺.
Agid:
5159378