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The effect of ions on the adhesion and internalization of Chlamydia trachomatis by HeLa cells

Sneddon, John M., Wenman, Wanda M.
Canadian journal of microbiology 1985 v.31 no.4 pp. 371-374
Chlamydia trachomatis, adhesion, barium, calcium, calmodulin, cations, cobalt, glucose, iron, lanthanum, magnesium, manganese, potassium, sodium, strontium, verapamil, zinc
The adhesion and internalization of Chlamydia trachomatis by HeLa cells was unaffected by removal of K⁺, Mg²⁺, or glucose from the incubation medium, slightly reduced by removal of Na⁺, and significantly reduced by omission of Ca²⁺. Sr²⁺, Mg²⁺, and Mn²⁺ could replace Ca²⁺ in the adhesion but only Sr²⁺ supported internalization, and La³⁺, Co²⁺, Fe³⁺, Ba²⁺, and Zn²⁺ all reduced internalization more than adhesion. During initial infection there was no measurable difference in the uptake or release of ⁴⁵Ca²⁺ or ⁸⁶Rb⁺ between infected and noninfected HeLa monolayers. Infection was not prevented by pretreatment of the monolayers with the calcium channel blockers, verapamil, D600, and nitrendipine, or the calmodulin inhibitors, TMB-8 or trifluperazine. The results suggest that divalent cations are not essential for chlamydial infection but that the process of internalization is facilitated by the presence of cations, particularly Na⁺ and Ca²⁺.