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Birth weight alters the response to postnatal high-fat diet-induced changes in meat quality traits and skeletal muscle proteome of pigs

Liu, Jingbo, He, Jun, Yu, Jie, Mao, Xiangbing, Zheng, Ping, Huang, Zhiqing, Yu, Bing, Chen, Daiwen
The British journal of nutrition 2014 v.111 no.10 pp. 1738-1747
apoptosis, cell structures, diet, drip loss, energy, energy metabolism, gene expression regulation, glucose, growth and development, heat shock proteins, intramuscular fat, lipid metabolism, lipids, low birth weight, meat quality, phosphopyruvate hydratase, piglets, progeny, protein synthesis, proteome, proteomics, pyruvate kinase, skeletal muscle, slaughter, stress response, triose-phosphate isomerase, weaning
Low birth weight (LBW) exerts persistent effects on the growth and development of offspring. The present study was conducted to test the hypothesis that LBW alters the response of pigs to high-fat (HF) diet-induced changes in meat quality and skeletal muscle proteome. Normal-birth weight (NBW) and LBW piglets were fed a control diet or a HF diet from weaning to slaughter at 110 kg body weight. Most of the meat quality traits were influenced by LBW. Meat quality analysis revealed that LBW piglets had a greater ability to deposit intramuscular lipids than their heavier littermates when fed a HF diet. Increased shear force, lower pH₄₅ ₘᵢₙ and drip loss were observed in the skeletal muscle of LBW piglets compared with NBW piglets. Proteomic analysis revealed forty-six differentially expressed proteins in the skeletal muscle of LBW and NBW piglets fed the control diet or HF diet. These proteins play a central role in cell structure and motility, glucose and energy metabolism, lipid metabolism, and cellular apoptosis, as well as stress response. Of particular interest is the finding that LBW altered the response to HF diet-induced changes in the expression of proteins related to stress response (heat shock protein) and glucose and energy metabolism (pyruvate kinase, phosphoglycerate mutase, enolase and triosephosphate isomerase). Taken together, our findings revealed that the HF diet-induced changes in the expression of glucose and energy metabolism-related proteins varied between NBW and LBW piglets, which provides a possible mechanism to explain higher intramuscular fat store in LBW pigs when fed a HF diet.