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Differences in plasma metabolomics between sows fed dl-methionine and its hydroxy analogue reveal a strong association of milk composition and neonatal growth with maternal methionine nutrition

Zhang, Xiaoling, Li, Hao, Liu, Guangmang, Wan, Haifeng, Mercier, Yves, Wu, Caimei, Wu, Xiuqun, Che, Lianqiang, Lin, Yan, Xu, Shengyu, Tian, Gang, Chen, Daiwen, Wu, De, Fang, Zhengfeng
The British journal of nutrition 2015 v.113 no.4 pp. 585-595
acetates, body weight, citrates, cysteine, dairy protein, fatty acid-binding proteins, formates, glucose, glycerol, glycogen, glycoproteins, histidine, homocysteine, jejunum, lactic acid, lactose, landraces, lipids, lysine, messenger RNA, metabolism, metabolomics, methionine, milk fat, milk synthesis, myo-inositol, nuclear magnetic resonance spectroscopy, ornithine, piglets, sows, taurine, tyrosine, urea nitrogen, valine
The aim of the present study was to determine whether increased consumption of methionine as dl-methionine (DLM) or its hydroxy analogue dl-2-hydroxy-4-methylthiobutanoic acid (HMTBA) could benefit milk synthesis and neonatal growth. For this purpose, eighteen cross-bred (Landrace × Yorkshire) primiparous sows were fed a control (CON), DLM or HMTBA diet (n 6 per diet) from 0 to 14 d post-partum. At postnatal day 14, piglets in the HMTBA group had higher body weight (P= 0·02) than those in the CON group, tended (P= 0·07) to be higher than those in the DLM group, and had higher (P< 0·05) mRNA abundance of jejunal fatty acid-binding protein 2, intestinal than those in the CON and DLM groups. Compared with the CON diet-fed sows, milk protein, non-fat solid, and lysine, histidine and ornithine concentrations decreased in the DLM diet-fed sows (P< 0·05), and milk fat, lactose, and cysteine and taurine concentrations increased in the HMTBA diet-fed sows (P< 0·05). Plasma homocysteine and urea N concentrations that averaged across time were increased (P< 0·05) in sows fed the DLM diet compared with those fed the CON diet. Metabolomic results based on ¹H NMR spectroscopy revealed that consumption of the HMTBA and DLM diets increased (P< 0·05) both sow plasma methionine and valine levels; however, consumption of the DLM diet led to lower (P< 0·05) plasma levels of lysine, tyrosine, glucose and acetate and higher (P< 0·05) plasma levels of citrate, lactate, formate, glycerol, myo-inositol and N-acetyl glycoprotein in sows. Collectively, neonatal growth and milk synthesis were regulated by dietary methionine levels and sources, which resulted in marked alterations in amino acid, lipid and glycogen metabolism.