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A food-grade fimbrial adhesin FaeG expression system in Lactococcus lactis and Lactobacillus casei

Lu, W.W., Wang, T., Wang, Y., Xin, M., Kong, J.
Canadian journal of microbiology 2015 v.62 no.3 pp. 241-248
DNA, Lactobacillus casei, Lactococcus lactis, Staphylococcus aureus, Western blotting, adhesins, alanine, antigens, diarrhea, enterotoxigenic Escherichia coli, fimbriae, genes, lactic acid bacteria, pathogenesis, piglets, replicon, secretion, signal peptide, vaccination, vaccine development
Enterotoxigenic Escherichia coli (ETEC) infection is the major cause of diarrhea in neonatal piglets. The fimbriae as colonizing factor in the pathogenesis of ETEC constitute a primary target for vaccination against ETEC. Lactic acid bacteria (LAB) are attractive tools to deliver antigens at the mucosal level. With the safety of genetically modified LAB in mind, a food-grade secretion vector (pALRc or pALRb) was constructed with DNA entirely from LAB, including the replicon, promoter, signal peptide, and selection marker alanine racemase gene (alr). To evaluate the feasibility of the system, the nuclease gene (nuc) from Staphylococcus aureus was used as a reporter to be expressed in both Lactococcus lactis and Lactobacillus casei. Subsequently, the extracellular secretion of the fimbrial adhesin FaeG of ETEC was confirmed by Western blot analysis. These results showed that this food-grade expression system has potential as the delivery vehicle for the safe use of genetically modified LAB for the development of vaccines against ETEC infection.