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Supplementation of Chitosan Alleviates High-Fat Diet-Enhanced Lipogenesis in Rats via Adenosine Monophosphate (AMP)-Activated Protein Kinase Activation and Inhibition of Lipogenesis-Associated Genes

Chiu, Chen-Yuan, Chan, Im-Lam, Yang, Tsung-Han, Liu, Shing-Hwa, Chiang, Meng-Tsan
Journal of agricultural and food chemistry 2015 v.63 no.11 pp. 2979-2988
adenosine monophosphate, adipocytes, adipose tissue, biosynthesis, chitosan, diet, enzyme activity, epididymis, genes, hypertrophy, lipogenesis, lipolysis, lipoprotein lipase, liver, obesity, phosphorylation, rats, regulatory proteins, transcription factors, triacylglycerols
This study investigated the role of chitosan in lipogenesis in high-fat diet-induced obese rats. The lipogenesis-associated genes and their upstream regulatory proteins were explored. Diet supplementation of chitosan efficiently decreased the increased weights in body, livers, and adipose tissues in high-fat diet-fed rats. Chitosan supplementation significantly raised the lipolysis rate; attenuated the adipocyte hypertrophy, triglyceride accumulation, and lipoprotein lipase activity in epididymal adipose tissues; and decreased hepatic enzyme activities of lipid biosynthesis. Chitosan supplementation significantly activated adenosine monophosphate (AMP)-activated protein kinase (AMPK) phosphorylation and attenuated high-fat diet-induced protein expressions of lipogenic transcription factors (PPAR-γ and SREBP1c) in livers and adipose tissues. Moreover, chitosan supplementation significantly inhibited the expressions of downstream lipogenic genes (FAS, HMGCR, FATP1, and FABP4) in livers and adipose tissues of high-fat diet-fed rats. These results demonstrate for the first time that chitosan supplementation alleviates high-fat diet-enhanced lipogenesis in rats via AMPK activation and lipogenesis-associated gene inhibition.