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Development and Validation of a HPLC Method Using a Monolithic Column for Quantification of trans-Resveratrol in Lipid Nanoparticles for Intestinal Permeability Studies

Neves, Ana Rute, Reis, Salette, Segundo, Marcela A.
Journal of agricultural and food chemistry 2015 v.63 no.12 pp. 3114-3120
acetic acid, acetonitrile, ambient temperature, beverages, bioactive properties, fluorometry, freeze-thaw cycles, functional foods, high performance liquid chromatography, in vitro studies, lipids, nanoparticles, permeability
The development of nanodelivery systems that protect trans-resveratrol is extremely important to preserve its bioactive properties in the development of further applications as nutraceuticals to supplement foods and beverages. In this work, a validated HPLC method was developed for the quantification of trans-resveratrol in lipid nanoparticles for application in studies of in vitro intestinal permeability. The chromatographic separation was achieved in a C18 monolithic column connected to a fluorometric detector (330/374 nm), by isocratic elution consisting of 2% acetic acid/acetonitrile (80:20). Two calibration ranges were established (0.020–0.200 and 0.200–2.00 μmol L–1), and low quantification limits (2–6 nmol L–1, 23–69 pg) were achieved. Stability studies showed that trans-resveratrol is stable for 24 h at 4 °C, and storage at room temperature and freeze–thaw cycles are not recommended. The proposed method was applied to in vitro intestinal permeability studies, in which values between 0.05 ± 0.01 and 1.8 ± 0.3 μmol L–1 were found.