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Patch clamp characterization of a non-selective cation channel of ER membranes purified from Beta vulgaris taproots

Author:
Brito-Argáez, Ligia, Canto-Canché, Blondy, Teresa Hernández-Sotomayor, Soledad Maria, Martínez-Estevez, Manuel, Pottosin, Igor I.
Source:
Physiologia plantarum 2008 v.132 no.4 pp. 399-406
ISSN:
0031-9317
Subject:
Beta vulgaris, beets, cations, lipids, permeability, sucrose
Abstract:
The ER fraction from red beet taproot was purified on sucrose gradient and giant liposomes, suitable for patch clamping, were formed by dehydration-rehydration of the lipid film. Single-channel recordings on excised and attached patches revealed a large conductance (165 pS) cation (PCl₋/PK₊ < 0.03) channel with equal conductance and relative permeability for Na⁺ and K⁺. This non-selective cation channel was also highly permeable for Ca²⁺. We failed to detect any single-channel currents activated by a direct application of d-myo-inositol 1,4,5 trisphosphate, despite the fact that the ER membranes were native.
Agid:
517147