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The utility of yeast as a tool for cell-based, target-directed high-throughput screening

NORCLIFFE, J. L., ALVAREZ-RUIZ, E., MARTIN-PLAZA, J. J., STEEL, P. G., DENNY, P. W., Barrett, Michael P., Croft, Simon L.
Parasitology 2014 v.141 no.1 pp. 8-16
cell culture, cost effectiveness, parasites, screening, yeasts
Many Neglected Tropical Diseases (NTDs) have recently been subject of increased focus, particularly with relation to high-throughput screening (HTS) initiatives. These vital endeavours largely rely of two approaches, in vitro target-directed screening using biochemical assays or cell-based screening which takes no account of the target or targets being hit. Despite their successes both of these approaches have limitations; for example, the production of soluble protein and a lack of cellular context or the problems and expense of parasite cell culture. In addition, both can be challenging to miniaturize for ultra (u)HTS and expensive to utilize. Yeast-based systems offer a cost-effective approach to study and screen protein targets in a direct-directed manner within a eukaryotic cellular context. In this review, we examine the utility and limitations of yeast cell-based, target-directed screening. In particular we focus on the currently under-explored possibility of using such formats in uHTS screening campaigns for NTDs.