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Effects of low-density lipoproteins extracted from different avian yolks on boar spermatozoa quality following freezing–thawing

Wang, Peng, Wang, Yan-Feng, Wang, Chun-Wei, Bu, Shu-Hai, Hu, Jian-Hong, Li, Qing-Wang, Pang, Wei-Jun, Yang, Gong-She
Zygote 2014 v.22 no.2 pp. 175-181
acrosome, boars, cryoprotectants, duck eggs, egg yolk, eggs, freeze-thaw cycles, freezing, hens, low density lipoprotein, ostriches, pigeons, plasma membrane, quails, semen quality, sperm motility, sperm quality
Low-density lipoproteins (LDL) is known to protect boar sperm during freezing–thawing, but little information is known about the effects of LDL extracted from different avian egg yolks on post-thaw boar semen quality. The purpose of this study was to compare and analyze the effects of LDL at various concentrations and different species on boar sperm quality after freezing–thawing. LDL extracted from the yolk of hen egg, duck egg, quail egg, pigeon egg or ostrich egg was added to the extender at the concentrations of 0.06, 0.07, 0.08, 0.09 and 0.1 g/ml, respectively, and their effects on frozen–thawed boar sperm quality were assessed. According to all measured parameters, the results showed that sperm motility, acrosome integrity and plasma membrane integrity were 43.20%, 52.57% and 48.13%, respectively, after being frozen–thawed with 0.09 g/ml LDL extracted from pigeon egg yolk. All these quality parameters were higher than that of other groups (P < 0.05). In conclusion, our results confirmed that LDL extracted from pigeon egg yolk had the best cryoprotective effects on frozen–thawed boar sperm among all of the groups supplemented with LDL from five kinds of avian egg in extender. The optimum concentration of LDL extracted from pigeon egg in boar semen freezing extender was 0.09 g/ml.