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Fast and Accurate Screening of Solanum melongena with High-Resolution Melting Analysis for Resistance to Fusarium Wilt

Ganopoulos, Ioannis, Xanthopoulou, Aliki, Konstantinou, Sotiris, Karaoglanidis, George S., Tsaliki, Eleni, Kalivas, Apostolos, Madesis, Panagiotis
International journal of vegetable science 2016 v.22 no.2 pp. 183-189
Fusarium oxysporum, Fusarium wilt, Solanum melongena, breeding, cultivars, eggplants, genetic background, germplasm, germplasm evaluation, landraces, melting, screening, virulent strains
The causal agent of Fusarium wilt, Fusarium oxysporum Schlecht. f. sp. melongenae , produces a vascular disease of eggplant (Solanum melongena L.). Resistant germplasm with wide variation is essential for controlling this disease via breeding, and understanding the genetic background helps design breeding strategies. This study screened 36 S. melongena germplasm landraces and cultivars for Fusarium wilt resistance. Fusarium oxysporum is a particularly complex species composed of several pathogenic strains arranged into groups called formae speciales, which pose a challenge to developing resistant plant varieties. Screening and selecting resistant plant sources could play an important role in developing resistant or tolerant plant lines. High-resolution melting analysis coupled with a sequenced characterized amplified region marker for fast screening and accurate identification of Fusarium wilt–resistant eggplant has been developed. The method could be useful in breeding where great numbers of plants need to be tested during germplasm evaluation and for single plant selection. Breeders could use these findings to choose Fusarium wilt–resistant cultivars/landraces for their improvement.