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Sequence and comparative analysis of the MIP gene in Chinese straw mushroom, Volvariella volvacea

Chen, Bing-Zhi, Gui, Fu, Xie, Bao-Gui, Zou, Feng, Jiang, Yu-Ji, Deng, You-Jin
Genome 2012 v.55 no.9 pp. 667-672
DNA, Volvariella volvacea, computer software, fungi, genes, nucleotide sequences, phylogeny
The mitochondrial intermediate peptidase (MIP) gene is conserved in fungi. It is linked closely with the mating-type A (mtA) gene. In this study, a fragment of the MIP gene in Volvariella volvacea (Bull. ex Fr.) Singer was first cloned by homologue-based cloning technology. Subsequently, the entire MIP DNA sequence (PYd21-MIP) was obtained after the fragment was compared with the genomic data through BLAST analysis. The PYd21-MIP sequence appeared to be homologous with the MIP gene in other fungi. Phylogenetic analysis of PYd21-MIP and other MIP sequences from diverse fungi agreed with the current organism phylogeny. Analysis of protein domains by InterProScan software and motif searching demonstrated that PYd21-MIP encodes a homologous MIP protein. These data support the hypothesis that the PYd21-MIP protein is a Hog-MIP protein homologue from V. volvacea.