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Characterization of wheat – Secale africanum introgression lines reveals evolutionary aspects of chromosome 1R in rye

Lei, Meng-Ping, Li, Guang-Rong, Liu, Cheng, Yang, Zu-Jun
Genome 2012 v.55 no.10 pp. 765-774
Secale cereale, chromosome banding, chromosome translocation, chromosomes, disease resistance, domestication, evolution, gene duplication, genes, genetic markers, in situ hybridization, introgression, nucleotide sequences, races, rye, screening, stripe rust, wheat
Wild Secale species, Secale africanum Stapf., serve as a valuable source for increasing the diversity of cultivated rye (Secale cereale L.) and provide novel genes for wheat improvement. New wheat – S. africanum chromosome 1Rᵃᶠʳ addition, 1Rᵃᶠʳ(1D) substitution, 1BL.1RᵃᶠʳS and 1DS.1RᵃᶠʳL translocation, and 1RᵃᶠʳL monotelocentric addition lines were identified by chromosome banding and in situ hybridization. Disease resistance screening revealed that chromosome 1RᵃᶠʳS carries resistance gene(s) to new stripe rust races. Twenty-nine molecular markers were localized on S. africanum chromosome 1Rᵃᶠʳ by the wheat – S. africanum introgression lines. Twenty markers can also identically amplify other reported wheat – S. cereale chromosome 1R derivative lines, indicating that there is high conservation between the wild and cultivated Secale chromosome 1R. Nine markers displayed polymorphic amplification between S. africanum and S. cereale chromosome 1Rᵃᶠʳ derivatives. The comparison of the nucleotide sequences of these polymorphic markers suggested that gene duplication and sequence divergence may have occurred among Secale species during its evolution and domestication.