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Characterization of wheat – Psathyrostachys huashanica small segment translocation line with enhanced kernels per spike and stripe rust resistance

Kang, Hou-Yang, Zhang, Zhi-Juan, Xu, Li-Li, Qi, Wei-Liang, Tang, Yao, Wang, Hao, Zhu, Wei, Li, Dai-Yan, Zeng, Jian, Wang, Yi, Fan, Xing, Sha, Li-Na, Zhang, Hai-Qin, Zhou, Yong-Hong
Genome 2016 v.59 no.4 pp. 221-229
Psathyrostachys huashanica, breeding, centromeres, chromosome translocation, cultivars, disease resistance, fluorescence in situ hybridization, genes, genetic improvement, germplasm, growing season, inflorescences, meiosis, pathogens, seeds, stripe rust, translocation lines, wheat, China
Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs), a distant wild relative of common wheat, possesses rich potentially valuable traits, such as disease resistance and more spikelets and kernels per spike, that could be useful for wheat genetic improvement. Development of wheat – P. huashanica translocation lines will facilitate its practical utilization in wheat breeding. In the present study, a wheat – P. huashanica small segmental translocation line, K-13-835-3, was isolated and characterized from the BC₁F₅ population of a cross between wheat – P. huashanica amphiploid PHW-SA and wheat cultivar CN16. Cytological studies showed that the mean chromosome configuration of K-13-835-3 at meiosis was 2n = 42 = 0.10 I + 19.43 II (ring) + 1.52 II (rod). GISH analyses indicated that chromosome composition of K-13-835-3 included 40 wheat chromosomes and a pair of wheat – P. huashanica translocation chromosomes. FISH results demonstrated that the small segment from an unidentified P. huashanica chromosome was translocated into wheat chromosome arm 5DS, proximal to the centromere region of 5DS. Compared with the cultivar wheat parent CN16, K-13-835-3 was highly resistant to stripe rust pathogens prevalent in China. Furthermore, spikelets and kernels per spike in K-13-835-3 were significantly higher than those of CN16 in two growing seasons. These results suggest that the desirable genes from P. huashanica were successfully transferred into CN16 background. This translocation line could be used as novel germplasm for high-yield and, eventually, resistant cultivar breeding.