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Factors affecting fructosyltransferases and fructan exohydrolase activities in Agave tequilana Weber var. azul

García-Pérez, María Concepción, López, Mercedes G.
Journal of plant biochemistry and biotechnology 2016 v.25 no.2 pp. 147-154
Agave tequilana, crops, deoxycholic acid, fructans, fructose, kestose, magnesium chloride, mercuric chloride, metabolism, micropropagation, nystose, polymers, silver nitrate, sodium dodecyl sulfate
There is great interest in the fructosyltransferases (FTFs) involved in fructan metabolism and agents affecting their activity. Agaves accumulate fructans, fructose polymers linked by glycosidic β(2–1) and β(2–6) bonds in linear or branched configurations. In plants, fructans provide protection under stress conditions. The sucrose:sucrose 1-fructosyltransferase (1-SST), fructan:fructan 1-fructosyltransferase (1-FFT), fructan:fructan 6G-fructosyltransferase (6G-FFT), and fructan exohydrolase (FEH) activities were analyzed in micropropagated Agave tequilana plants in the absence and presence of HgCl₂, AgNO₃, MgCl₂, sodium deoxycholate (DNa), and sodium dodecyl sulfate (SDS). Kestose, nystose and neokestose were synthesized by the respective FTFs. HgCl₂ and AgNO₃ inhibited all FTFs, mainly up to 90 % in 1-SST and 1-FFT. DNa increased 1-SST (32 %) and 1-FFT (45 %) activities, and SDS increased 6G-FFT activity by 96 %. Finally, AgNO₃ inhibited FEH activity by 78 %. Our results might be relevant on the regulation of FTFs in agave and other crops, for instance by the increment the fructans synthesis in stressed plants.