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PCR for the Specific Detection of an Escherichia coli O157:H7 Laboratory Control Strain

Knowles, Michael, Lambert, Dominic, Huszcynski, George, Gauthier, Martine, Blais, Burton W.
Journal of food protection 2015 v.78 no.9 pp. 1738-1744
DNA primers, Escherichia coli O157, bacteria, bioinformatics, food microbiology, pathogens, polymerase chain reaction, quality control, risk management
Control strains of bacterial pathogens such as Escherichia coli O157:H7 are commonly processed in parallel with test samples in food microbiology laboratories as a quality control measure to assure the satisfactory performance of materials used in the analytical procedure. Before positive findings can be reported for risk management purposes, analysts must have a means of verifying that pathogenic bacteria (e.g., E. coli O157:H7) recovered from test samples are not due to inadvertent contamination with the control strain routinely handled in the laboratory environment. Here, we report on the application of an in-house bioinformatic pipeline for the identification of unique genomic signature sequences in the development of specific oligonucleotide primers enabling the identification of a common positive control strain, E. coli O157:H7 (ATCC 35150), using a simple PCR procedure.