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Metabolism of the Fusarium Mycotoxins T-2 Toxin and HT-2 Toxin in Wheat

Nathanail, Alexis V., Varga, Elisabeth, Meng-Reiterer, Jacquelin, Bueschl, Christoph, Michlmayr, Herbert, Malachova, Alexandra, Fruhmann, Philipp, Jestoi, Marika, Peltonen, Kimmo, Adam, Gerhard, Lemmens, Marc, Schuhmacher, Rainer, Berthiller, Franz
Journal of agricultural and food chemistry 2015 v.63 no.35 pp. 7862-7872
Fusarium, Fusarium head blight, HT-2 toxin, T-2 toxin, Triticum aestivum, acetylation, biotransformation, cultivars, isotope labeling, mass spectrometry, metabolism, metabolites, metabolomics, ripening, wheat
To investigate the metabolic fate of HT-2 toxin (HT2) and T-2 toxin (T2) in wheat (Triticum aestivum L.), an untargeted metabolomics study utilizing stable isotopic labeling and liquid chromatography–high resolution mass spectrometry was performed. In total, 11 HT2 and 12 T2 derived in planta biotransformation products were annotated putatively. In addition to previously reported mono- and diglucosylated forms of HT2, evidence for the formation of HT2-malonyl-glucoside and feruloyl-T2, as well as acetylation and deacetylation products in wheat was obtained for the first time. To monitor the kinetics of metabolite formation, a time course experiment was conducted involving the Fusarium head blight susceptible variety Remus and the resistant cultivar CM-82036. Biotransformation reactions were observed already at the earliest tested time point (6 h after treatment), and formed metabolites showed different kinetic profiles. After ripening, less than 15% of the toxins added to the plants were determined to be unmetabolized.