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Residual polymerase activity-induced bias in terminal restriction fragment length polymorphism analysis
- Hartmann, Martin, Enkerli, Jürg, Widmer, Franco
- Environmental microbiology 2007 v.9 no.2 pp. 555-559
- polymerase chain reaction, restriction fragment length polymorphism, soil ecology
- Residual activity of polymerase chain reaction DNA polymerases in restriction analyses strongly affected genetic profiling based on terminal restriction fragment length polymorphisms. Artificial fragment sizes produced as a result of 5'-overhang restriction site fill-in and addition of a terminal A may bias genetic profiling and genotyping of microbial communities. Efficient removal of polymerases retained original fragment sizes and significantly reduced this profiling bias in soil bacterial communities.