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Residual polymerase activity-induced bias in terminal restriction fragment length polymorphism analysis

Author:
Hartmann, Martin, Enkerli, Jürg, Widmer, Franco
Source:
Environmental microbiology 2007 v.9 no.2 pp. 555-559
ISSN:
1462-2912
Subject:
polymerase chain reaction, restriction fragment length polymorphism, soil ecology
Abstract:
Residual activity of polymerase chain reaction DNA polymerases in restriction analyses strongly affected genetic profiling based on terminal restriction fragment length polymorphisms. Artificial fragment sizes produced as a result of 5'-overhang restriction site fill-in and addition of a terminal A may bias genetic profiling and genotyping of microbial communities. Efficient removal of polymerases retained original fragment sizes and significantly reduced this profiling bias in soil bacterial communities.
Agid:
518417