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Medium Perfusion in Cultures of Small Aquatic Animals

Author:
Salt, George W.
Source:
Ecology 1965 v.46 no.1-2 pp. 208-209
ISSN:
0012-9658
Subject:
Protozoa, animal experimentation, animals, aquatic organisms, capillarity, drying, glass, metabolites
Abstract:
For experiments on animal populations which run for extended periods of time, a method of removal of medium is needed in order to avoid accumulation of metabolites and inhibition of population processes. This can be done for cultures of small aquatic organisms, such as protozoa, by using a filter paper wick which removes fluid by capillary action. The animals are not removed. A like volume of fresh fluid is added after each removal to maintain a constant volume. To prevent drying of the wick, the culture dish and wick are kept in a saturated atmosphere inside a glass cabinet or box.
Agid:
5184406