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Cloning of TaSST genes associated with water soluble carbohydrate content in bread wheat stems and development of a functional marker

Author:
Dong, Yan, Zhang, Yan, Xiao, Yonggui, Yan, Jun, Liu, Jindong, Wen, Weie, Zhang, Yong, Jing, Ruilian, Xia, Xianchun, He, Zhonghu
Source:
Theoretical and applied genetics 2016 v.129 no.5 pp. 1061-1070
ISSN:
0040-5752
Subject:
Triticum aestivum, alleles, biochemical pathways, breeding, carbohydrate content, chromosomes, cultivars, exons, fructans, genetic distance, genetic markers, inbred lines, quantitative trait loci, sequence analysis, single nucleotide polymorphism, stems, wheat, China
Abstract:
KEY MESSAGE: We cloned TaSST genes, developed a gene-specific marker for TaSST - D1 , and identified three QTL in the Doumai/Shi 4185 RIL population. TaSST - D1 is within one of the three QTL. Sucrose:sucrose-1-fructosyltransferase (1-SST), a critical enzyme in the fructan biosynthetic pathway, is significantly and positively associated with water soluble carbohydrate (WSC) content in bread wheat stems. In the present study, wheat 1-SST genes (TaSST) were isolated and located on chromosomes 4A, 7A and 7D. Sequence analysis of TaSST-D1 revealed 15 single nucleotide polymorphisms (SNP) in the third exon between cultivars with higher and lower WSC content. A cleaved amplified polymorphism sequence (CAPS) marker, WSC7D, based on the polymorphism at position 1216 (C-G) was developed to discriminate the two alleles. WSC7D was located on chromosome 7DS using a recombinant inbred line (RIL) population from a Doumai/Shi 4185 cross, and a set of Chinese Spring nullisomic-tetrasomic lines. TaSST-D1 co-segregated with the CAPS marker WSC7D and was linked to SNP marker BS00108793_51 on chromosome 7DS at a genetic distance of 6.1 cM. It explained 8.8, 10.9, and 11.3 % of the phenotypic variances in trials at Beijing and Shijiazhuang as well as the averaged data from those environments, respectively. Two additional QTL (QWSC.caas-4BS and QWSC.caas-7AS) besides TaSST-D1 were mapped in the RIL population. One hundred and forty-nine Chinese wheat cultivars and advanced lines tested in four environments were used to validate a highly significant (P < 0.01) association between WSC7D and WSC content in wheat stems. WSC7D can be used as a gene-specific marker for improvement of stem WSC content in wheat breeding programs.
Agid:
5187645