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Cellulosic hydrogen production with a sequencing bacterial hydrolysis and dark fermentation strategy

Lo, Yung-Chung, Bai, Ming-Der, Chen, Wen-Ming, Chang, Jo-Shu
Bioresource technology 2008 v.99 no.17 pp. 8299-8303
Clostridium, agitation, bagasse, carboxymethylcellulose, cellulose, culture media, fermentation, hydrogen production, hydrolysates, hydrolysis, pH, reducing sugars, rice hulls
In this study, cellulose hydrolysis activity of two mixed bacterial consortia (NS and QS) was investigated. Combination of NS culture and BHM medium exhibited better hydrolytic activity under the optimal condition of 35°C, initial pH 7.0, and 100rpm agitation. The NS culture could hydrolyze carboxymethyl cellulose (CMC), rice husk, bagasse and filter paper, among which CMC gave the best hydrolysis performance. The CMC hydrolysis efficiency increased with increasing CMC concentration from 5 to 50g/l. With a CMC concentration of 10g/l, the total reducing sugar (RS) production and the RS producing rate reached 5531.0mg/l and 92.9mg/l/h, respectively. Furthermore, seven H₂-producing bacterial isolates (mainly Clostridium species) were used to convert the cellulose hydrolysate into H₂ energy. With an initial RS concentration of 0.8g/l, the H₂ production and yield was approximately 23.8ml/l and 1.21mmol H₂/g RS (0.097mmol H₂/g cellulose), respectively.