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Rice OsVAMP714, a membrane-trafficking protein localized to the chloroplast and vacuolar membrane, is involved in resistance to rice blast disease

Author:
Sugano, Shoji, Hayashi, Nagao, Kawagoe, Yasushi, Mochizuki, Susumu, Inoue, Haruhiko, Mori, Masaki, Nishizawa, Yoko, Jiang, Chang-Jie, Matsui, Minami, Takatsuji, Hiroshi
Source:
Plant molecular biology 2016 v.91 no.1-2 pp. 81-95
ISSN:
0167-4412
Subject:
Magnaporthe oryzae, Oryza sativa, amino acids, animals, blast disease, cell membranes, chloroplasts, disease resistance, fungi, gene overexpression, hyphae, immunity, leaves, mesophyll, mutants, pathogens, physiological transport, proteins, rice, transgenic plants, vacuoles, vegetative growth
Abstract:
Membrane trafficking plays pivotal roles in many cellular processes including plant immunity. Here, we report the characterization of OsVAMP714, an intracellular SNARE protein, focusing on its role in resistance to rice blast disease caused by the fungal pathogen Magnaporthe oryzae. Disease resistance tests using OsVAMP714 knockdown and overexpressing rice plants demonstrated the involvement of OsVAMP714 in blast resistance. The overexpression of OsVAMP7111, whose product is highly homologous to OsVAMP714, did not enhance blast resistance to rice, implying a potential specificity of OsVAMP714 to blast resistance. OsVAMP714 was localized to the chloroplast in mesophyll cells and to the cellular periphery in epidermal cells of transgenic rice plant leaves. We showed that chloroplast localization is critical for the normal OsVAMP714 functioning in blast resistance by analyzing the rice plants overexpressing OsVAMP714 mutants whose products did not localize in the chloroplast. We also found that OsVAMP714 was located in the vacuolar membrane surrounding the invasive hyphae of M. oryzae. Furthermore, we showed that OsVAMP714 overexpression promotes leaf sheath elongation and that the first 19 amino acids, which are highly conserved between animal and plant VAMP7 proteins, are crucial for normal rice plant growths. Our studies imply that the OsVAMP714-mediated trafficking pathway plays an important role in rice blast resistance as well as in the vegetative growth of rice.
Agid:
5190714