PubAg

Main content area

Separation of three phenolic high‐molecular‐weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound‐assisted extraction and high‐speed counter‐current chromatography

Author:
Zou, Deng‐lang, Chen, Tao, Li, Hong‐mei, Chen, Chen, Zhao, Jing‐yang, Li, Yu‐lin
Source:
Journal of separation science 2016 v.39 no.7 pp. 1278-1285
ISSN:
1615-9306
Subject:
Terminalia chebula, acetates, countercurrent chromatography, ellagic acid, methanol, molecular weight, nuclear magnetic resonance spectroscopy, phenolic compounds, response surface methodology, solvents, ultrasonic treatment
Abstract:
This study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound‐assisted extraction and high‐speed counter‐current chromatography. The ultrasound‐assisted extraction conditions were optimized by response surface methodology and the results showed the target compounds could be well enriched under the optimized extraction conditions. Then the crude extract was directly separated by high‐speed counter‐current chromatography without any pretreatment using n‐hexane/ethyl acetate/methanol/water (1:7:0.5:3, v/v/v/v) as the solvent system. In 180 min, 13 mg of A, 18 mg of B, and 9 mg of C were obtained from 200 mg of crude sample. Their structures were identified as Chebulagic acid (A, 954 Da), Chebulinic acid (B, 956 Da), and Ellagic acid (C) by ¹H NMR spectroscopy.
Agid:
5191716