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Isoliensinine, a Bioactive Alkaloid Derived from Embryos of Nelumbo nucifera, Induces Hepatocellular Carcinoma Cell Apoptosis through Suppression of NF-κB Signaling

Author:
Shu, Guangwen, Yue, Ling, Zhao, Wenhao, Xu, Chan, Yang, Jing, Wang, Shaobing, Yang, Xinzhou
Source:
Journal of agricultural and food chemistry 2015 v.63 no.40 pp. 8793-8803
ISSN:
0021-8561
Subject:
Nelumbo nucifera, alkaloids, apoptosis, blood, food plants, hepatoma, intraperitoneal injection, mice, neoplasm cells, phosphorylation, tissues, toxicity, transcription factor NF-kappa B
Abstract:
Isoliensinine (isolie) is an alkaloid produced by the edible plant Nelumbo nucifera. Here, we unveiled that isolie was able to provoke HepG2, Huh-7, and H22 hepatocellular carcinoma (HCC) cell apoptosis. Isolie decreased NF-κB activity and constitutive phosphorylation of NF-κB p65 subunit at Ser536 in HCC cells. Overexpression of p65 Ser536 phosphorylation mimics abrogated isolie-mediated HCC cell apoptosis. Furthermore, intraperitoneal injection of isolie inhibited the growth of Huh-7 xenografts in nude mice. Additionally, isolie given by both intraperitoneal injection and gavage diminished the proliferation of transplanted H22 cells in Kunming mice. Reduced tumor growth in vivo was associated with inhibited p65 phosphorylation at Ser536 and declined NF-κB activity in tumor tissues. Finally, we revealed that isolie was bioavailable in the blood of mice and exhibited no detectable toxic effects on tumor-bearing mice. Our data provided strong evidence for the anti-HCC effect of isolie.
Agid:
5192224