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Comparison between solid-state and powder-state alkali pretreatment on saccharification and fermentation for bioethanol production from rice straw
- Yeasmin, Shabina, Kim, Chul-Hwan, Islam, Shah Md. Asraful, Lee, Ji-Young
- Preparative biochemistry & biotechnology 2016 v.46 no.3 pp. 229-237
- Fourier transform infrared spectroscopy, absorbance, alkali treatment, atomic force microscopy, biomass, ethanol, ethanol fermentation, ethanol production, glucose, hydrolysis, lignin, rice straw, saccharification, scanning electron microscopy, sodium hydroxide
- The efficacy of different concentrations of NaOH (0.25%, 0.50%, 0.75%, and 1.00%) for the pretreatment of rice straw in solid and powder state in enzymatic saccharification and fermentation for the production of bioethanol was evaluated. A greater amount of biomass was recovered through solid-state pretreatment (3.74 g) from 5 g of rice straw. The highest increase in the volume of rice straw powder as a result of swelling was observed with 1.00% NaOH pretreatment (48.07%), which was statistically identical to 0.75% NaOH pretreatment (32.31%). The surface of rice straw was disrupted by the 0.75% NaOH and 1.00% NaOH pretreated samples as observed using field-emission scanning electron microscopy (FE-SEM) and atomic force microscopy (AFM). In Fourier-transform infrared (FT-IR) spectra, absorbance of hydroxyl groups at 1,050 cm ⁻¹ due to the OH group of lignin was gradually decreased with the increase of NaOH concentration. The greatest amounts of glucose and ethanol were obtained in 1.00% NaOH solid-state pretreated and powder-state hydrolyzed samples (0.804 g g ⁻¹ and 0.379 g g ⁻¹, respectively), which was statistically similar to the use of 0.75% NaOH (0.763 g g ⁻¹ and 0.358 g g ⁻¹, respectively). Thus, solid-state pretreatment with 0.75% NaOH and powder-state hydrolysis appear to be suitable for fermentation and bioethanol production from rice straw.