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Role of the flavonoid-rich fraction in the antioxidant and cytotoxic activities of Bauhinia forficata Link. (Fabaceae) leaves extract

Miceli, Natalizia, Buongiorno, Luigina Pasqualina, Celi, Maria Grazia, Cacciola, Francesco, Dugo, Paola, Donato, Paola, Mondello, Luigi, Bonaccorsi, Irene, Taviano, Maria Fernanda
Natural product research 2016 v.30 no.11 pp. 1229-1239
Artemia salina, Bauhinia forficata, anti-infective properties, antioxidant activity, bacteria, cell growth, cell viability, cytotoxicity, diabetic complications, flavonoids, free radical scavengers, humans, leaf extracts, lymphocytes, neoplasms, oxidative stress, yeasts
Bauhinia forficata Link. is utilised as an antidiabetic in Brazilian folk-medicine; furthermore, its antioxidant properties suggest a potential usefulness in the prevention of diabetes complications associated with oxidative stress. The contribution of a flavonoid-rich fraction (FRF), HPLC-PDA-ESI-MS characterised, to the antioxidant and cytotoxic properties of B. forficata hydro-alcoholic leaves extract was evaluated for the first time. Both extract and FRF showed radical-scavenging activity and reducing power with a strong relationship with the flavonoid content found; hence, flavonoids are mainly responsible for the primary antioxidant activity of B. forficata extract. The extract significantly decreased FO-1 cell viability at the higher concentrations. FRF did not exert any effect; thus, flavonoids do not appear to be responsible for the cytotoxicity of the extract. The extract resulted virtually non-toxic against both Artemia salina and normal human lymphocytes, demonstrating potential selectivity in inhibiting cancer cell growth. Finally, no antimicrobial activity was observed against the bacteria and yeasts tested.