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Role of the flavonoid-rich fraction in the antioxidant and cytotoxic activities of Bauhinia forficata Link. (Fabaceae) leaves extract
- Miceli, Natalizia, Buongiorno, Luigina Pasqualina, Celi, Maria Grazia, Cacciola, Francesco, Dugo, Paola, Donato, Paola, Mondello, Luigi, Bonaccorsi, Irene, Taviano, Maria Fernanda
- Natural product research 2016 v.30 no.11 pp. 1229-1239
- Artemia salina, Bauhinia forficata, anti-infective properties, antioxidant activity, bacteria, cell growth, cell viability, cytotoxicity, diabetic complications, flavonoids, free radical scavengers, humans, leaf extracts, lymphocytes, neoplasms, oxidative stress, yeasts
- Bauhinia forficata Link. is utilised as an antidiabetic in Brazilian folk-medicine; furthermore, its antioxidant properties suggest a potential usefulness in the prevention of diabetes complications associated with oxidative stress. The contribution of a flavonoid-rich fraction (FRF), HPLC-PDA-ESI-MS characterised, to the antioxidant and cytotoxic properties of B. forficata hydro-alcoholic leaves extract was evaluated for the first time. Both extract and FRF showed radical-scavenging activity and reducing power with a strong relationship with the flavonoid content found; hence, flavonoids are mainly responsible for the primary antioxidant activity of B. forficata extract. The extract significantly decreased FO-1 cell viability at the higher concentrations. FRF did not exert any effect; thus, flavonoids do not appear to be responsible for the cytotoxicity of the extract. The extract resulted virtually non-toxic against both Artemia salina and normal human lymphocytes, demonstrating potential selectivity in inhibiting cancer cell growth. Finally, no antimicrobial activity was observed against the bacteria and yeasts tested.