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Identification of ALV-J associated acutely transforming virus Fu-J carrying complete v-fps oncogene
- Wang, Yixin, Li, Jianliang, Li, Yang, Fang, Lichun, Sun, Xiaolong, Chang, Shuang, Zhao, Peng, Cui, Zhizhong
- Virus genes 2016 v.52 no.3 pp. 365-371
- Avian leukosis virus, carcinogenicity, crossbreds, evolution, oncogenes, polymerase chain reaction, sequence analysis, viruses, China
- Transduction of oncogenes by ALVs and generation of acute transforming viruses is common in natural viral infections. In order to understand the molecular basis for the rapid oncogenicity of Fu-J, an acutely transforming avian leukosis virus isolated from fibrosarcomas in crossbreed broilers infected with subgroup J avian leukosis virus (ALV-J) in China, complete genomic structure of Fu-J virus was determined by PCR amplification and compared with those of Fu-J1, Fu-J2, Fu-J3, Fu-J4, and Fu-J5 reported previously. The results showed that the genome of Fu-J was defective, with parts of gag gene replaced by the complete v-fps oncogene and encoded a 137 kDa Gag-fps fusion protein. Sequence analysis revealed that Fu-J and Fu-J1 to Fu-J5 were related quasi-species variants carrying different lengths of v-fps oncogenes generated from recombination between helper virus and c-fps gene. Comparison of virus carrying v-fps oncogene also gave us a glimpse of the molecular characterization and evolution process of the acutely transforming ALV.