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Screening bioactive components affecting the capacity of bile acid binding and pancreatic lipase inhibitory activity

Author:
Truong, Ngoc-Hoa, Lee, Suyong, Shim, Soon-Mi
Source:
Han'guk Ŭngyong Saengmyŏng Hwahakhoe chi = 2016 v.59 no.3 pp. 475-479
Subject:
apigenin, ascorbic acid, beta-glucans, bile acids, binding capacity, bioactive compounds, butyrates, cellulose, cholestyramine, curcumin, dietary fiber, digestion, enzyme activity, enzyme inhibition, enzyme inhibitors, hesperidin, humans, hydrolysis, hydrophobicity, kaempferol, micelles, pectins, polyphenols, quercetin, screening, toxicity, triacylglycerol lipase, water solubility
Abstract:
The hypothesis of the current study was that bioactive components including vitamins, dietary fibers, and polyphenols may have both the ability to bind to bile acid and inhibit pancreatic lipase activity. ß-carotene, vitamin C, and vitamin U as vitamin, cellulose, ß-glucan, and pectin as dietary fibers, and apigenin, curcumin, hesperidin, kaempferol, and quercetin as polyphenols were chosen. The bile acid binding capacity was measured via the furfural–H₂SO₄ test at the concentration of 2.5 mg/mL with cholestyramine resin used as a positive control (binding value of 87.17 %). The pancreatic lipase inhibitory ability of bioactive components was measured by following the hydrolysis of p-nitrophenyl butyrate. Among the compounds tested, pectin, vitamin C, and ß-glucan had the highest binding capacity to bile acid, at 82.7, 67.8, and 33 %, respectively. The results suggest a positive correlation between water solubility and the bile acid binding capacity of the tested bioactive components. At 20 mg/mL concentration, the inhibiting values of all components were relatively lower than the positive control (orlistat, 54.4 %), ranging from 1.3 to 7.6 % of the negative control. Among them, curcumin and cellulose are the highest pancreatic lipase inhibitors with inhibiting values of 7.6 and 7.5 %, respectively. These results imply ß-glucan, pectin, vitamin C, cellulose, and curcumin as potential components in reducing the uptake of hydrophobic toxic components through inhibiting micelle formation under human digestion fluid.
Agid:
5200532