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Development of microsatellite markers for Manilkara maxima T.D. Penn. (Sapotaceae) and their use in conservation genetics

Silva-Junior, José Audenor, de Souza França, Daniele, Moraes, Ramiris César Souza, Gaiotto, Fernanda Amato
Molecular biology reports 2016 v.43 no.6 pp. 451-455
Manilkara, alleles, conservation programs, ecology, endangered species, forests, genetic markers, genetic variation, heterozygosity, high-throughput nucleotide sequencing, indigenous species, kinship, loci, microsatellite repeats, paternity, population genetics, probability, trees, Brazil
Manilkara maxima is an endemic tree species of the Atlantic Forest in southern Bahia, Brazil. It is considered important for forest conservation due to its mutualistic interactions with endemic and endangered animals. Our aim was to develop microsatellite markers to estimate genetic diversity in order to provide information for effectiveness of future conservation programs. We used next generation sequencing technology to develop the first specific microsatellite markers for M. maxima. Seventeen new microsatellite loci were applied in 72 individuals sampled in three natural populations. On average, the number of alleles per loci was 8.8. The expected heterozygosity varied between 0.72 and 0.77, indicating that the developed set of molecular markers is useful for genetic diversity studies. Additionally, the estimated value for the combined probability of exclusion (Q) was greater than 0.999, which indicates the powerful of these molecular tools for paternity and kinship analysis. Our results demonstrate that the set of microsatellites developed in this work is a powerful tool for population genetics, molecular ecology and conservation biology purposes.