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Optimization of the Antioxidant Extraction from Eleutherococcus Senticosus Roots by Response Surface Methodology

Ondrejovič, Miroslav, Chmelová, Daniela, Maliar, Tibor
Nova biotechnologica et chimica 2013 v.12 no.1 pp. 30-38
2,2-diphenyl-1-picrylhydrazyl, Eleutherococcus senticosus, antioxidant activity, antioxidants, ethanol, health promotion, polyphenols, response surface methodology, roots, solvents, temperature
Eleutherococcus senticosus is known as adaptogen with benefits in general health promotion. The aim of this study was to investigate the effect of major extraction parameters on extraction yield of antioxidants measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity. Secondly, content of total polyphenols was evaluated. Optimal conditions of the extraction were processed by response surface methodology. The independent variables of extraction were proposed as temperature, solid-liquid ratio and solvent composition. For the optimal antioxidant extraction, E. senticosus is suitable to extract by 23 % (v/v) aqueous ethanol at 70 °C in ratio 53 mL of extraction solvent per g of plant material. The optimal conditions calculated for the extraction of total polyphenols were very similar (70 °C, 22 % (v/v) aqueous ethanol) expect solid-liquid ratio which indicates need of increasing of solid-liquid ratio to 91 mL of extraction solvent per g of plant material