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Structural Characterization and Antioxidant Activity of a Novel Alkaline-Soluble Polysaccharide from Patrinia villosa
- He, L., Han, S. F., Cheng, J. W., Wang, Y. B., Ding, H. M., Wei, C. Y., Qian, H.
- Chemistry of natural compounds 2016 v.52 no.3 pp. 384-387
- 2,2-diphenyl-1-picrylhydrazyl, Patrinia villosa, anion exchange, antioxidant activity, galactose, gas chromatography, gel chromatography, glucose, hydroxyl radicals, inhibitory concentration 50, mannose, methylation, nuclear magnetic resonance spectroscopy, polysaccharides, sodium hydroxide
- A novel alkaline-soluble polysaccharide (PVPS-3), obtained from Patrinia villosa by 9.0% NaOH extraction, was fractionated by anion-exchange and gel filtration chromatography. Using Fourier infrared spectrometry, gas chromatography, methylation, and NMR, we determined PVPS-3 to be a 2.61 kDa heteropolysaccharide composed of L-arabinose, D-mannose, D-glucose, and D-galactose in a ratio of 2.3:1.1:4.9:1.2. Purified PVPS-3 showed concentration-dependent scavenging activity of 2,2-diphenyl-1-picrylhydrazyl radical and moderate hydroxyl radical scavenging (IC₅₀ 1.5 mg/mL) compared with crude polysaccharide (IC₅₀ 2.3 mg/mL). PVPS-3 is promising as a potential antioxidant.