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Determination of Tetracycline Antibiotic Residues in Honey and Milk by Miniaturized Solid Phase Extraction Using Chitosan-Modified Graphitized Multiwalled Carbon Nanotubes

Xu, Jing-Jing, An, Mingrui, Yang, Rui, Tan, Zhijing, Hao, Jie, Cao, Jun, Peng, Li-Qing, Cao, Wan
Journal of agricultural and food chemistry 2016 v.64 no.12 pp. 2647-2654
acetic acid, acetonitrile, antibiotic residues, carbon nanotubes, detection limit, food matrix, honey, mass spectrometry, milk, solid phase extraction, solvents, tetracycline, ultra-performance liquid chromatography
A rapid, simple, and strongly selective miniaturized solid phase extraction (SPE) technique, requiring only small amounts of sorbent (24 mg) and elution solvent (600 μL), coupled with ultrahigh-performance liquid chromatography and quadrupole time-of-flight mass spectrometry was developed for detecting tetracycline antibiotics. These analytes were extracted from honey and milk using chitosan-modified graphitized multiwalled carbon nanotubes (G-MWNTs) as the solid sorbent and acetonitrile/acetic acid (8:2, v/v) as the eluent in miniaturized SPE. Under the optimum experimental conditions, a satisfactory linearity (r2 > 0.992) was obtained, and the limits of detection were in the range of 0.61–10.34 μg/kg for the analytes. The mean recoveries of the five tetracycline antibiotic residues in the real samples were between 81.5 and 101.4%. The results demonstrated that chitosan-modified G-MWNTs comprise a promising material for the enrichment of tetracycline antibiotics from complex food matrices.