Jump to Main Content
Notizen: Improved 2,4-Dinitrophenylhydrazine, Thin Layer Chromatography Methods for the Determination of Micro- and Macroamounts of Ascorbic Acid
- Navon, A., Levinson, H. Z.
- Zeitschrift für Naturforschung 2014 v.29 no.11-12 pp. 777-780
- absorbance, acetic acid, aqueous solutions, ascorbic acid, condensation reactions, dehydroascorbic acid, glass fibers, solvents, thin layer chromatography
- Microamounts of vitamin C could be readily determined in 20 μl-samples using the 2,4-dinitrophenylhydrazine method together with separation by thin layer chromatography. The condensation reaction was carried out for 5 min at 100 °C on a glass fibre disc. Purification of vitamin C hydrazones was accomplished by repeated separation on TLC plates. An aqueous solution of 65% acetic acid was employed to dissolve the vitamin C hydrazones, providing maximal absorbance at 500 nm. The minimum amount detectable by this method is 0.4 μg of dehydroascorbic acid. The macrodetermination of vitamin C was improved by simplifying a previous method and employing 65% aqueous acetic acid as a solvent for the hydrazones.