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Photosynthetic Hydrogen Evolution by Spinach Chloroplasts Coupled to a Clostridium Hydrogenase
- Hoffmann, D., Thauer, R., Trebst, A.
- Zeitschrift für Naturforschung 2014 v.32 no.3-4 pp. 257-262
- Clostridium, Spinacia oleracea, chlorophyll, hydrogen, hydrogen production, photosystem I, spinach, thylakoids
- Photosystem I activity of isolated chloroplasts has been coupled to photosynthetic hydrogen evolution via methylviologen and a Clostridium hydrogenase. Rates of 125 μmol hydrogen evolved per mg chlorophyll and hour have been obtained. The conditions for such optimal activity suggest that a closed thylakoid vesicle is required in order to prevent side-and backreactions of reduced methylviologen, which compete with and reduce hydrogen evolution.