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The Degree of CF1 Release and the Reconstituting Capacity of the Depleted Membranes
- Schopf, Reinhard
- Zeitschrift für Naturforschung 2014 v.32 no.7-8 pp. 600-604
- Spinacia oleracea, cations, chlorophyll, photophosphorylation, spinach, thylakoids
- Uncoupling of photophosphorylation was shown in chloroplasts from spinach which were iso lated in media containing pyrophosphate and thereafter suspended in salt-free solutions. The effective concentration of pyrophosphate to uncouple was determined by the addition of pyrophos phate to free thylakoid systems and shown to be dependent on the presence of the completely dissociated species which favoured complex formation with bivalent cations. Higher concentrations of pyrophosphate led to a stabilization of photophosphorylation. The uncoupling is due to the release of the coupling factor. The degree of liberation was shown to be dependent on the chlorophyll concentration. The capacity of extracted membranes to reconstitute photophosphorylation with their corresponding supernatant or with purified CF₁ was directly correlated to the amount of the liberated coupling factor.