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Evidence for Uptake of Plamid DNA into Intact Plants (Lemna perpusilla) Proved by an E. coli Transformation Assay

Author:
Frey, M., Rail, S., Roth, A., Hemleben, V.
Source:
Zeitschrift für Naturforschung 2014 v.35 no.11-12 pp. 1104-1106
ISSN:
1865-7125
Subject:
Escherichia coli, Lemna aequinoctialis, plasmids, tetracycline
Abstract:
The water plant Lemna perpusilla has been incubated with the E. coli plasmids pMB9 and pBR325, respectively. Uptake of plasmids has been shown by subsequent trans­ formation of E. coli cells to tetracycline resistance after treatment with Lemna DNA from plasmid-incubated plants. In 7 out of 15 assays we found stable transformants. From the transformation rate an amount of 10⁻⁴ to 10⁻⁶ μg plasmid DNA per 10 μg of plant DNA can be calculated.
Agid:
5226325