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Absorption Spectra of Phytochrome Intermediates
- Eilfeld, Peter, Rüdiger, Wolfhart
- Zeitschrift für Naturforschung 2014 v.40 no.1-2 pp. 109-114
- Avena sativa, absorption, etiolation, far-red light, phytochrome, seedlings, temperature
- Native phytochrome (124 kdalton) was isolated from etiolated seedlings of Avena sativa L. cv. Pirol (Baywa, Munich). From low temperature spectra (0°C to - 165 °C) of phytochrome samples irradiated either with red or far-red light, spectra of intermediates were calculated. Both absorption bands of the phytochrome chromophore were reported. On the pathway Pᵣ → Pfᵣ, native phytochrome does not form a bleached intermediate (meta-Rb) in contrast to degraded phytochrome.An additional intermediate, meta-Rc (λₘₐₓ = 725 nm), is formed instead. On the reverse pathway only two intermediates were found, lumi-F and meta-F. Oscillator strengths′ values were calculated and interpreted in terms of chromophore conformation. A scheme of intermediates for native phytochrome is presented.