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Enzymatic Nitrate Assay by a Kinetic Method Employing Escherichia coli Nitrate Reductase

Author:
Schild, Johannes, Klemme, Jobst-Heinrich
Source:
Zeitschrift für Naturforschung 2014 v.40 no.1-2 pp. 134-137
ISSN:
0939-5075
Subject:
Escherichia coli, nitrate reductase, nitrates
Abstract:
An enzymatic assay system for nitrate employing the membrane-bound nitrate reductase (EC 1.7.99.4) of E. coli is described. Contrary to previous enzymatic assay systems, the present method is a kinetic one, i.e. the substrate, nitrate, is assayed by measuring the reaction rate of the nitrate reductase-catalyzed reaction. Based on the observation that the nitrate reductase-catalyzed reaction obeys pseudo-first order kinetics, a test system is described allowing the assay of nitrate at a concentration as low as 1 ppm. The relatively high M ichaelis-M enten constant for nitrate (0.3 mᴍ) of the E. coli nitrate reductase favours nitrate assay by the kinetic method.
Agid:
5227358