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Metabolie Conversions of Trichothecene Mycotoxins: De-esterification Reactions Using Cell-Free Extracts of Fusarium
- Udell, Michael N., Dewick, Paul M.
- Zeitschrift für Naturforschung 2014 v.44 no.7-8 pp. 660-668
- 3-acetyldeoxynivalenol, Fusarium, deoxynivalenol, enzyme activity, esterases, esters
- A crude cell-free extract from cultures of Fusarium sp. strain C37410-90 possessed significant esterase activity and hydrolyzed the trichothecene mycotoxin 3-acetyldeoxynivalenol (3-AcDON) to deoxynivalenol (DON) in high yield. Smaller amounts of 15-acetyl- and 3,15-diacetyl-esters of DON were also formed. The extract was capable of hydrolyzing a range of natural and semisynthetic trichothecene esters, and showed a high degree of regioselectivity towards position 3.3,4,15-Triacetylscirpentriol (TAS) was efficiently hydrolyzed to 4,5-diacetoxyscirpenol (DAS), and no further transformation was observed. The enzyme activity was partially purified.