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Effect of Azide on the ATPase Activity of Isolated CF1
- Minkov, Ignat B., Strotmann, Heinrich
- Zeitschrift für Naturforschung 2014 v.46 no.7-8 pp. 621-628
- Ca2-transporting ATPase, Spinacia oleracea, adenosinetriphosphatase, chloroplasts, enzyme activation, magnesium, spinach
- The effect of azide on M g²⁺-and Ca²⁺-dependent ATPase of differently activated CF₁ isolated from spinach chloroplasts was studied. It is shown that Mg²⁺-ATPase activity is sensitive towards azide irrespective of the applied method of enzyme activation. Heat-or trypsin-acti-vated Ca²⁺-ATPase is also inhibited, whereas methanol-and octylglucoside-stimulated or DTT-activated Ca²⁺-ATPase is not affected by azide. Preincubation of the DTT-activated enzyme with low concentrations of Mg²⁺ induces azide susceptibility of the Ca²⁺-dependent ATPase.